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Status |
Public on Oct 31, 2017 |
Title |
GFP positive, biological rep1 |
Sample type |
RNA |
|
|
Source name |
adult zebrafish (GFP-positive) sorted cone photoreceptors
|
Organism |
Danio rerio |
Characteristics |
age: adult genotype/variation: Tg(3.2TalphaCP:EGFP) tissue: cone cells cell population: GFP positive
|
Treatment protocol |
No treatment was done prior to zebrafish euthanasia
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Growth protocol |
Zebrafish were kept in transparent 10-liter aquaria, heated to 23ºC-28ºC degrees with a pH between 6.2 to 7.5. Normally, 25 fish were kept in each tank. Fish were maintained with a 14-hour light / 10-hour dark cycle. Fish were fed with ground dry trout pellets, zooplankton, phytoplankton and insect larvae twice a day.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated with the RNeasy Micro Kit (Qiagen) protocol (Mack et al 2007) in order to increase the final RNA concentration
|
Label |
Biotin
|
Label protocol |
Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
|
|
Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
Description |
GFPp-1
|
Data processing |
The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
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|
|
Submission date |
Aug 29, 2016 |
Last update date |
Oct 31, 2017 |
Contact name |
Breandan Kennedy |
E-mail(s) |
brendan.kennedy@ucd.ie
|
Organization name |
UCD Conway Institute, University College Dublin
|
Department |
UCD School of Biomolecular and Biomedical Science
|
Lab |
Kennedy Lab
|
Street address |
Belfield
|
City |
Dublin |
ZIP/Postal code |
Dublin 4 |
Country |
Ireland |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE86155 |
IDENTIFICATION OF ADULT ZEBRAFISH CONE PHOTORECEPTOR-ENRICHED GENES |
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