|
| Status |
Public on Oct 31, 2017 |
| Title |
GFP minus, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
adult zebrafish (GFP-minus) sorted cone photoreceptors
|
| Organism |
Danio rerio |
| Characteristics |
age: adult
genotype/variation: Tg(3.2TalphaCP:EGFP)
tissue: cone cells
cell population: GFP minus
|
| Treatment protocol |
No treatment was done prior to zebrafish euthanasia
|
| Growth protocol |
Zebrafish were kept in transparent 10-liter aquaria, heated to 23ºC-28ºC degrees with a pH between 6.2 to 7.5. Normally, 25 fish were kept in each tank. Fish were maintained with a 14-hour light / 10-hour dark cycle. Fish were fed with ground dry trout pellets, zooplankton, phytoplankton and insect larvae twice a day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Micro Kit (Qiagen) protocol (Mack et al 2007) in order to increase the final RNA concentration
|
| Label |
Biotin
|
| Label protocol |
Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
| Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
| Description |
GFPm-3
|
| Data processing |
The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
|
| |
|
| Submission date |
Aug 29, 2016 |
| Last update date |
Oct 31, 2017 |
| Contact name |
Breandan Kennedy |
| E-mail(s) |
brendan.kennedy@ucd.ie
|
| Organization name |
UCD Conway Institute, University College Dublin
|
| Department |
UCD School of Biomolecular and Biomedical Science
|
| Lab |
Kennedy Lab
|
| Street address |
Belfield
|
| City |
Dublin |
| ZIP/Postal code |
Dublin 4 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE86155 |
IDENTIFICATION OF ADULT ZEBRAFISH CONE PHOTORECEPTOR-ENRICHED GENES |
|
