|
| Status |
Public on Feb 01, 2017 |
| Title |
Nephron progenitors replicate #1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
FACS isolated nephron progenitors at E14 replicate #1
|
| Organism |
Mus musculus |
| Characteristics |
strain: Six2-TGC
tissue: FACS isolated nephron progenitors at E14 replicate #1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen miRNeasy kit, as per manufacturer's directions
|
| Label |
Cy3
|
| Label protocol |
miRCURY Hy3/Hy5 power labeling kit, done by Exiqon
|
| |
|
| Channel 2 |
| Source name |
Embryonic day 14 kidney
|
| Organism |
Mus musculus |
| Characteristics |
strain: Six2-TGC
tissue: Embryonic day 14 whole kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen miRNeasy kit, as per manufacturer's directions
|
| Label |
Cy5
|
| Label protocol |
miRCURY Hy3/Hy5 power labeling kit, done by Exiqon
|
| |
|
| |
| Hybridization protocol |
Done by Exiqon
|
| Scan protocol |
Done by Exiqon
|
| Data processing |
The quantified signals were normalized using the global Lowess (Locally Weighted Scatterplot Smoothing) regression algorithm, done by Exiqon
|
| |
|
| Submission date |
Aug 30, 2016 |
| Last update date |
Feb 01, 2017 |
| Contact name |
Jacqueline Ho |
| E-mail(s) |
jacqueline.ho2@chp.edu
|
| Phone |
412-692-5303
|
| Organization name |
Children's Hospital of Pittsburgh
|
| Department |
Pediatrics
|
| Street address |
4401 Penn Avenue
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15224 |
| Country |
USA |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE86217 |
miRNA expression in nephron progenitors of the developing kidney |
|
