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Sample GSM2298856 Query DataSets for GSM2298856
Status Public on Nov 30, 2017
Title Heart_untreated_iCMAKT1+2 KO_10_d_rep3
Sample type RNA
 
Source name Heart_untreated_iCMAKT1+2 KO_10_d
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6
induction: Tamoxifen
time: 10d after Tamoxifen induction
genotype/variation: AKT1+AKT2 DKO
tissue: Heart apex
Treatment protocol Mice were treated with 4-OH-Tamoxifen (5 injections, 100 µl each, one injection per day) to induce the knockout. No further treatments to modify e.g. cardiac function were applied.
Growth protocol na
Extracted molecule total RNA
Extraction protocol RNA was isolated using Fibrous Tissue RNeasy Kit, Qiagen according to the manufacturers protocol.
Label Cy3
Label protocol Synthesis of cDNA and subsequent fluorescent labelling of cRNA was performed according to the manufacturers´ protocol (One-Color Microarray-Based Gene Expression Analysis / Low Input Quick Amp Labeling; Agilent Technologies). Briefly, 100 ng of total RNA were converted to cDNA, followed by in vitro transcription and incorporation of Cy3-CTP into nascent cRNA.
 
Hybridization protocol After fragmentation labelled cRNA was hybridized to Agilent SurePrint G3 Mouse GE 8x60K Microarrays for 17 h at 65 °C.
Scan protocol Microarrays were scanned as described in the manufacturers´ protocol. Signal intensities on 20 bit tiff images were calculated by Feature Extraction software (FE, Vers. 11.0.1.1; Agilent Technologies).
Description 9703
Transgene: heterozygous:MHC-mercremer, homozygous AKT1+AKT2 flox/flox
Data processing Probe signal intensities were quantile normalized across all samples to reduce inter-array variability. Input data pre-processing was concluded by baseline transformation to the median of all samples.
 
Submission date Aug 30, 2016
Last update date Nov 30, 2017
Contact name Karl Koehrer
E-mail(s) rene.deenen@hhu.de
Organization name University of Duesseldorf
Department BMFZ
Lab GTL
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
 
Platform ID GPL21163
Series (1)
GSE86230 Effect of cardiomyocyte specific KO of AKT1 + AKT2 on gene expression profile of the heart

Data table header descriptions
ID_REF
VALUE Normalized signal intensity; log2

Data table
ID_REF VALUE
GE_BrightCorner -0.11677265
DarkCorner -0.08002639
A_51_P399985 -0.22625732
A_55_P2508138 -0.19813204
A_55_P2805880 0.100734234
A_55_P2419483 -0.42834044
A_55_P2739683 -0.27236748
A_51_P211903 -0.09197426
A_66_P121325 0
A_51_P226429 -0.069312096
A_55_P2841743 -0.12608242
A_55_P2737159 0.1582098
A_55_P2728466 -0.060403824
A_55_P2101526 -0.72364616
A_52_P1132414 -1.1048226
A_66_P135936 0.44030952
A_55_P2805396 0.049432755
A_55_P2717104 -0.012683392
A_55_P2909714 0
A_55_P2744310 0

Total number of rows: 56745

Table truncated, full table size 1311 Kbytes.




Supplementary file Size Download File type/resource
GSM2298856_9703_US45103065_257480910485_S01_GE1_1200_Jun14_1_2.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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