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Sample GSM2298867 Query DataSets for GSM2298867
Status Public on Nov 30, 2017
Title Heart_untreated_iCMAKT2 KO_rep3
Sample type RNA
 
Source name Heart_untreated_iCMAKT2 KO
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6
induction: Tamoxifen
time: 21d after Tamoxifen induction
genotype/variation: AKT2 KO
tissue: Heart apex
Treatment protocol Mice were treated with 4-OH-Tamoxifen (5 injections, 100 µl each, one injection per day) to induce the knockout. No further treatments to modify e.g. cardiac function were applied.
Growth protocol na
Extracted molecule total RNA
Extraction protocol RNA was isolated using Fibrous Tissue RNeasy Kit, Qiagen according to the manufacturers protocol.
Label Cy3
Label protocol Synthesis of cDNA and subsequent fluorescent labelling of cRNA was performed according to the manufacturers´ protocol (One-Color Microarray-Based Gene Expression Analysis / Low Input Quick Amp Labeling; Agilent Technologies). Briefly, 100 ng of total RNA were converted to cDNA, followed by in vitro transcription and incorporation of Cy3-CTP into nascent cRNA.
 
Hybridization protocol After fragmentation labelled cRNA was hybridized to Agilent SurePrint G3 Mouse GE 8x60K Microarrays for 17 h at 65 °C.
Scan protocol Microarrays were scanned as described in the manufacturers´ protocol. Signal intensities on 20 bit tiff images were calculated by Feature Extraction software (FE, Vers. 11.0.1.1; Agilent Technologies).
Description M42_1572
Transgene: heterozygous:MHC-mercremer, homozygous AKT2 flox/flox
Data processing Probe signal intensities were quantile normalized across all samples to reduce inter-array variability. Input data pre-processing was concluded by baseline transformation to the median of all samples.
 
Submission date Aug 30, 2016
Last update date Nov 30, 2017
Contact name Karl Koehrer
E-mail(s) rene.deenen@hhu.de
Organization name University of Duesseldorf
Department BMFZ
Lab GTL
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
 
Platform ID GPL21163
Series (1)
GSE86231 Effect of cardiomyocyte specific KO of AKT1, AKT2, and AKT1 + AKT2 on gene expression profile of the heart

Data table header descriptions
ID_REF
VALUE Normalized signal intensity; log2

Data table
ID_REF VALUE
GE_BrightCorner 0.04684162
DarkCorner 0.27649474
A_51_P399985 -0.12592316
A_55_P2508138 -0.024579763
A_55_P2805880 0.007069588
A_55_P2419483 0.29298687
A_55_P2739683 0.06687832
A_51_P211903 -0.027469635
A_66_P121325 0.0303998
A_51_P226429 -0.032687187
A_55_P2841743 -0.035654783
A_55_P2737159 -0.056772232
A_55_P2728466 0.024664879
A_55_P2101526 0.46078205
A_52_P1132414 0.45583892
A_66_P135936 0.027805328
A_55_P2805396 0.01688099
A_55_P2717104 -0.04132223
A_55_P2909714 0.13668442
A_55_P2744310 -0.16658115

Total number of rows: 56745

Table truncated, full table size 1399 Kbytes.




Supplementary file Size Download File type/resource
GSM2298867_M42_1572_US45103065_257480910484_S01_GE1_1200_Jun14_1_3.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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