|
| Status |
Public on Nov 30, 2017 |
| Title |
Heart_untreated_iCMAKT1+2 KO_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Heart_untreated_iCMAKT1+2 KO
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57Bl/6
induction: Tamoxifen
time: 21d after Tamoxifen induction
genotype/variation: AKT1+AKT2 DKO
tissue: Heart apex
|
| Treatment protocol |
Mice were treated with 4-OH-Tamoxifen (5 injections, 100 µl each, one injection per day) to induce the knockout. No further treatments to modify e.g. cardiac function were applied.
|
| Growth protocol |
na
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Fibrous Tissue RNeasy Kit, Qiagen according to the manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Synthesis of cDNA and subsequent fluorescent labelling of cRNA was performed according to the manufacturers´ protocol (One-Color Microarray-Based Gene Expression Analysis / Low Input Quick Amp Labeling; Agilent Technologies). Briefly, 100 ng of total RNA were converted to cDNA, followed by in vitro transcription and incorporation of Cy3-CTP into nascent cRNA.
|
| |
|
| Hybridization protocol |
After fragmentation labelled cRNA was hybridized to Agilent SurePrint G3 Mouse GE 8x60K Microarrays for 17 h at 65 °C.
|
| Scan protocol |
Microarrays were scanned as described in the manufacturers´ protocol. Signal intensities on 20 bit tiff images were calculated by Feature Extraction software (FE, Vers. 11.0.1.1; Agilent Technologies).
|
| Description |
M30_1677
Transgene: heterozygous:MHC-mercremer, homozygous AKT1+AKT2 flox/flox
|
| Data processing |
Probe signal intensities were quantile normalized across all samples to reduce inter-array variability. Input data pre-processing was concluded by baseline transformation to the median of all samples.
|
| |
|
| Submission date |
Aug 30, 2016 |
| Last update date |
Nov 30, 2017 |
| Contact name |
Karl Koehrer |
| E-mail(s) |
rene.deenen@hhu.de
|
| Organization name |
University of Duesseldorf
|
| Department |
BMFZ
|
| Lab |
GTL
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE86231 |
Effect of cardiomyocyte specific KO of AKT1, AKT2, and AKT1 + AKT2 on gene expression profile of the heart |
|
