|
Status |
Public on Dec 08, 2016 |
Title |
Cortex from non diabetic BKS db/m mouse 1 |
Sample type |
RNA |
|
|
Source name |
Cortex from nondiabetic littermate control mouse
|
Organism |
Mus musculus |
Characteristics |
tissue: Cortex disease state: non-diabetic
|
Treatment protocol |
None
|
Growth protocol |
None
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy Mini Kit (Qiagen, Hilden, Germany)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
|
|
Hybridization protocol |
Standard Affymetrix Protocol
|
Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
Description |
Gene expression data from nondiabetic db/m Cortex (24 weeks old)
|
Data processing |
The data were analyzed using RMA method and Mouse Entrez Gene custom CDF annotation version 10 was used for the normalization
|
|
|
Submission date |
Aug 31, 2016 |
Last update date |
Dec 08, 2016 |
Contact name |
Viji Nair |
E-mail(s) |
vijin@med.umich.edu
|
Organization name |
University of Michigan
|
Street address |
1152 West Medical Centre Dr.
|
City |
Ann Arbor |
ZIP/Postal code |
48331 |
Country |
USA |
|
|
Platform ID |
GPL7546 |
Series (1) |
GSE86300 |
Tissue-specific metabolic reprogramming drives nutrient flux in diabetic complications |
|