|
| Status |
Public on Dec 08, 2016 |
| Title |
Cortex from Diabetic BKS db/db mouse 2 |
| Sample type |
RNA |
| |
|
| Source name |
Cortex from Diabetic mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cortex
disease state: diabetic
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Mini Kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from Diabetic db/db Cortex (24 weeks old)
|
| Data processing |
The data were analyzed using RMA method and Mouse Entrez Gene custom CDF annotation version 10 was used for the normalization
|
| |
|
| Submission date |
Aug 31, 2016 |
| Last update date |
Dec 08, 2016 |
| Contact name |
Viji Nair |
| E-mail(s) |
vijin@med.umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL7546 |
| Series (1) |
| GSE86300 |
Tissue-specific metabolic reprogramming drives nutrient flux in diabetic complications |
|
