|
Status |
Public on Mar 12, 2008 |
Title |
0h Activin A control |
Sample type |
RNA |
|
|
Source name |
human embryonic stem cells
|
Organism |
Homo sapiens |
Characteristics |
line H1
|
Treatment protocol |
hES cells grown in N2B27-based chemically defined medium.
|
Growth protocol |
N2B27 medium contained DMEM/F12 (Hyclone), N2 and B27 supplements (Invitrogen), 0.5% BSA (fraction V, Invitrogen), non-essential amino acids, glutamine, pen/strep (Invitrogen), 0.1 mM ß-mercaptoethanol, and 20 ng/ml FGF2 (Peprotech).
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 400ng of total RNA; IVT: 7h
|
|
|
Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degrees Celsius
|
Scan protocol |
Using an Illumina scanner; gain factor: 2
|
Description |
Non-stimulated control (0h timepoint) in an Activin A-driven differentiation timecourse experiment
|
Data processing |
In BeadStudio 1.5, biological replicates were grouped, background subtracted and normalised using the "rank invariant" algorithm. Value = "25" denotes expression below ca. 50% of the detection threshold.
|
|
|
Submission date |
Sep 17, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Boris Greber |
E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
Organization name |
Max Planck Institute for Molecular Biomedicine
|
Department |
Cell and Developmental Biology
|
Street address |
Röntgenstraße 20
|
City |
Münster |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL2700 |
Series (2) |
GSE9063 |
Activin A-driven differentiation timecourse with human embryonic stem cells |
GSE9092 |
Human embryonic stem cells: media/growth factor effects and differentiation timecourse |
|