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Sample GSM229978 Query DataSets for GSM229978
Status Public on Mar 12, 2008
Title 0h Activin A control
Sample type RNA
 
Source name human embryonic stem cells
Organism Homo sapiens
Characteristics line H1
Treatment protocol hES cells grown in N2B27-based chemically defined medium.
Growth protocol N2B27 medium contained DMEM/F12 (Hyclone), N2 and B27 supplements (Invitrogen), 0.5% BSA (fraction V, Invitrogen), non-essential amino acids, glutamine, pen/strep (Invitrogen), 0.1 mM ß-mercaptoethanol, and 20 ng/ml FGF2 (Peprotech).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
Label Cy3
Label protocol Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 400ng of total RNA; IVT: 7h
 
Hybridization protocol Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degrees Celsius
Scan protocol Using an Illumina scanner; gain factor: 2
Description Non-stimulated control (0h timepoint) in an Activin A-driven differentiation timecourse experiment
Data processing In BeadStudio 1.5, biological replicates were grouped, background subtracted and normalised using the "rank invariant" algorithm. Value = "25" denotes expression below ca. 50% of the detection threshold.
 
Submission date Sep 17, 2007
Last update date Aug 14, 2011
Contact name Boris Greber
E-mail(s) boris.greber@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Street address Röntgenstraße 20
City Münster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL2700
Series (2)
GSE9063 Activin A-driven differentiation timecourse with human embryonic stem cells
GSE9092 Human embryonic stem cells: media/growth factor effects and differentiation timecourse

Data table header descriptions
ID_REF
VALUE same as UNF_VALUE but with flagged values removed
UNF_VALUE normalised expression intensity

Data table
ID_REF VALUE UNF_VALUE
GI_10047089-S 25
GI_10047091-S 208 208
GI_10047093-S 1034 1034
GI_10047099-S 214 214
GI_10047103-S 5420 5420
GI_10047105-S 25
GI_10047121-S 25
GI_10047123-S 506 506
GI_10047133-A 25
GI_10047133-I 27 27
GI_10092578-S 25
GI_10092585-S 54 54
GI_10092596-S 237 237
GI_10092600-S 555 555
GI_10092602-S 25
GI_10092603-S 25
GI_10092611-A 116 116
GI_10092616-S 69 69
GI_10092618-S 711 711
GI_10092638-S 77 77

Total number of rows: 24354

Table truncated, full table size 472 Kbytes.




Supplementary file Size Download File type/resource
GSM229978.txt.gz 226.4 Kb (ftp)(http) TXT
GSM229978_1.txt.gz 225.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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