|
| Status |
Public on Mar 12, 2008 |
| Title |
72h ActA timepoint |
| Sample type |
RNA |
| |
|
| Source name |
human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
line H1
|
| Treatment protocol |
hES cells grown in N2B27-based chemically defined medium were treated for 72h with recombinant Activin A in the absense of FGF2
|
| Growth protocol |
N2B27 medium contained DMEM/F12 (Hyclone), N2 and B27 supplements (Invitrogen), 0.5% BSA (fraction V, Invitrogen), non-essential amino acids, glutamine, pen/strep (Invitrogen), 0.1 mM ß-mercaptoethanol, and 100 ng/ml Activin A (R&D).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 400ng of total RNA; IVT: 7h
|
| |
|
| Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degrees Celsius
|
| Scan protocol |
Using an Illumina scanner; gain factor: 2
|
| Description |
72h timepoint in an Activin A-driven differentiation timecourse experiment
|
| Data processing |
In BeadStudio 1.5, biological replicates were grouped, background subtracted and normalised using the "rank invariant" algorithm. Value = "25" denotes expression below ca. 50% of the detection threshold.
|
| |
|
| Submission date |
Sep 17, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2700 |
| Series (2) |
| GSE9063 |
Activin A-driven differentiation timecourse with human embryonic stem cells |
| GSE9092 |
Human embryonic stem cells: media/growth factor effects and differentiation timecourse |
|
