|
Status |
Public on Sep 19, 2007 |
Title |
cells stimulated with lipoprotein prep 2, biological rep3 |
Sample type |
RNA |
|
|
Source name |
Human THP-1 macrophages, stimulated with lipoprotein prep2
|
Organism |
Homo sapiens |
Characteristics |
Monocyte cell line, can be differentiated to macrophage, ATCC no. TIB-202
|
Treatment protocol |
Standard media was replaced with serum-free media containing 2% BSA, lipoproteins (in PBS) or the equivalent volume of PBS (controls) were added, incubaton was carried out for 24h, cells were then washed twice in PBS, scraped and stored at -80deg
|
Growth protocol |
Standard THP-1 cell media
|
Extracted molecule |
total RNA |
Extraction protocol |
By Rneasy system (Qiagen) according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA prepared as standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol EukGE-WS2v5_450
|
Scan protocol |
Arrays were scanned using an Affymetrix GeneChip® Scanner 3000
|
Description |
Gene expression data
|
Data processing |
dChip (build April 15, 2005) was used for normalization and modeling using the PM-only model, Control rep1 as baseline array
|
|
|
Submission date |
Sep 19, 2007 |
Last update date |
Jul 15, 2019 |
Contact name |
Silvio Zaina |
Organization name |
Rigshospitalet
|
Department |
Clinical Biochemistry
|
Street address |
Blegdamsvej 9
|
City |
COPENHAGEN |
ZIP/Postal code |
2100 |
Country |
Denmark |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE9101 |
Expression data in native lipoprotein-stimulated human THP-1 macrophages |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |
Reanalyzed by |
GSE134312 |