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Sample GSM230357 Query DataSets for GSM230357
Status Public on Sep 19, 2007
Title cells stimulated with lipoprotein prep 2, biological rep3
Sample type RNA
 
Source name Human THP-1 macrophages, stimulated with lipoprotein prep2
Organism Homo sapiens
Characteristics Monocyte cell line, can be differentiated to macrophage, ATCC no. TIB-202
Treatment protocol Standard media was replaced with serum-free media containing 2% BSA, lipoproteins (in PBS) or the equivalent volume of PBS (controls) were added, incubaton was carried out for 24h, cells were then washed twice in PBS, scraped and stored at -80deg
Growth protocol Standard THP-1 cell media
Extracted molecule total RNA
Extraction protocol By Rneasy system (Qiagen) according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA prepared as standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix protocol EukGE-WS2v5_450
Scan protocol Arrays were scanned using an Affymetrix GeneChip® Scanner 3000
Description Gene expression data
Data processing dChip (build April 15, 2005) was used for normalization and modeling using the PM-only model, Control rep1 as baseline array
 
Submission date Sep 19, 2007
Last update date Jul 15, 2019
Contact name Silvio Zaina
Organization name Rigshospitalet
Department Clinical Biochemistry
Street address Blegdamsvej 9
City COPENHAGEN
ZIP/Postal code 2100
Country Denmark
 
Platform ID GPL570
Series (1)
GSE9101 Expression data in native lipoprotein-stimulated human THP-1 macrophages
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE dChip model based expression indices
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
225207_at 158.18 P
1562236_at 4.59 A
1554485_s_at 107.29 P
213566_at 586.28 P
204959_at 169.74 P
203980_at 1518.45 P
209933_s_at 201.92 P
204995_at 40.93 P
218051_s_at 369.37 P
227190_at 133 P
228532_at 372.31 P
236539_at 37.44 P
205119_s_at 497.62 P
242714_at 373.44 P
206978_at 56.89 P
202973_x_at 412.04 P
217078_s_at 75.92 A
217047_s_at 370.5 P
212096_s_at 249.47 P
1553317_s_at 81.27 P

Total number of rows: 54675

Table truncated, full table size 1011 Kbytes.




Supplementary file Size Download File type/resource
GSM230357.CEL.gz 4.0 Mb (ftp)(http) CEL
GSM230357.EXP.gz 379 b (ftp)(http) EXP
Processed data included within Sample table

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