|
| Status |
Public on Sep 21, 2007 |
| Title |
6h estrogen treatment |
| Sample type |
RNA |
| |
|
| Source name |
myometrium
|
| Organism |
Rattus norvegicus |
| Characteristics |
SD, female 70 days
|
| Treatment protocol |
60 days old rats were ovariectomized a week prior of treatment, estradiol benzoate in 10ug/kg sc was given (or vehicle for control animals), 6 or 24 h after treatment uterine horns were collected, myometrium was quickly separated and flash frozen in liquid nitrogen. Tissues collected from 6 animals were pooled fro further analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
tissue was pulverized in liquid nitrogen and Trizol protocol was used for totalRNA extraction
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared with Gibco BRL SuperScript Choice system, and biotinylated cRNA by in vitro transcription using Ambion MegaScript T7kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Genechip Rat Genome U34A array. GeneChips were washed at the Affymetrix Fluidics Station 400 and stained with SAPE.
|
| Scan protocol |
GeneChips were scanned using GeneArray2500 scanner
|
| Description |
none
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 20, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Dora Agbas |
| E-mail(s) |
dagbas@kumc.edu
|
| Organization name |
University of Kansas Medical Center
|
| Department |
Physiology
|
| Lab |
Smithlab
|
| Street address |
3901 Rainbow blvd.
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE9117 |
Expression data from rat uterine myometrium -effect of estrogen |
|
