|
| Status |
Public on May 07, 2008 |
| Title |
ST-HSC_Control_Rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Short-term hematopoietic stem cells, control
|
| Organism |
Mus musculus |
| Characteristics |
Mouse model: MxCre+.Pbx1+/f on a C57BL/6 background
Age: 7.5 wks old
Tissue: Bone marrow ST-HSC (Lin-cKit+Sca1+CD34+CD135-)
|
| Treatment protocol |
7 poly (I:C) injections every other day, last injection 4 wks before sacrifice
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA from sorted cells was purified using Trizol and subjected to two rounds of amplification.
|
| Label |
biotin
|
| Label protocol |
GeneChip IVT Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
GeneChip Hybridization Kit (Affymetrix)
|
| Scan protocol |
Arrays were scanned with a Gene Chip Scanner 3000 (Affymetrix) running GCOS 1.1.1 software.
|
| Description |
See GeneChip Two-Cycle Target labeling assay protocol (Affymetrix)
|
| Data processing |
Scanned data were exported to dChip software for normalization, statistical analysis and heat mapping. A perfect-match/mismatch (PM/MM) model was used for the calculation of expression values.
|
| |
|
| Submission date |
Sep 27, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Francesca Ficara |
| E-mail(s) |
francesca.ficara@humanitasresearch.it
|
| Organization name |
CNR/Istituto Clinico Humanitas
|
| Street address |
via Manzoni 56
|
| City |
Rozzano |
| State/province |
MI |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE9189 |
Differentially regulated genes in normal LT-HSC vs ST-HSC |
| GSE9198 |
Long-term hematopoietic stem cells and the proto-oncogene Pbx1 |
|
| Relations |
| Reanalyzed by |
GSE119085 |
