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Sample GSM2327000 Query DataSets for GSM2327000
Status Public on Sep 05, 2017
Title FAM13AKOAir [Sample019]
Sample type RNA
 
Source name mouse lung
Organism Mus musculus
Characteristics genotype: FAM13AKO
treatment: Air
strain: C57BL6
Treatment protocol Mice were exposed to mixed main-stream and side-stream CS from 3R4F Kentucky Research cigarettes for 5 days/week for 6 months in Teague TE 10z chambers (TSP ~ 100-200 mg/m 3 and CO levels ~6 ppm).
Extracted molecule total RNA
Extraction protocol RNA was extracted from murine lung tissues using Allprep kit (Qiagen, Valencia, CA).
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol 750ng of total RNA for each sample was hybridized onto a Sentrix MouseRef-8 v2.0 Expression BeadChip Array (Illumina, San Diego, CA), based on the manufacturer's protocol.
Scan protocol The BeadChip Array was scanned using an Illumina BeadArray Reader and quality control analysis was performed using Illumina GenomeStudio v3.1.3 software, Gene Expression Module version 1.1.1, and the statistical package R v3.3.1.
Description FAM13AKOAir [Sample019]
Data processing Each of the samples and all probes passed quality control, and the data were background corrected, log2 transformed and quantile normalized.
 
Submission date Sep 23, 2016
Last update date Sep 05, 2017
Contact name Jeong H Yun
E-mail(s) jeong.yun@channing.harvard.edu
Organization name BRIGHAM AND WOMEN'S HOSPITAL
Street address 181 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6885
Series (1)
GSE87292 Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways

Data table header descriptions
ID_REF
VALUE quantile normalized
Sample019.Detection.Pval

Data table
ID_REF VALUE Sample019.Detection.Pval
ILMN_1250052 7.286001732 0.00251
ILMN_3122480 7.011300398 0.38847
ILMN_2599935 8.079769187 0
ILMN_2675543 6.9873131 0.5401
ILMN_2686883 7.253222084 0.00251
ILMN_2751818 6.996757747 0.47744
ILMN_2728634 7.213328699 0.00627
ILMN_3040515 7.167706109 0.01378
ILMN_2711608 7.307364854 0.00251
ILMN_1232875 7.177589339 0.00877
ILMN_1258507 7.039588354 0.24311
ILMN_2746142 6.995775304 0.48622
ILMN_1252690 7.069578363 0.15163
ILMN_2655499 7.006529095 0.42105
ILMN_1252870 7.021083525 0.33459
ILMN_1248179 6.960364203 0.71554
ILMN_2649955 7.469889013 0.00125
ILMN_2628708 7.212556366 0.00627
ILMN_3024781 6.981304262 0.57769
ILMN_2705628 8.896111929 0

Total number of rows: 25697

Table truncated, full table size 765 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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