NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2327014 Query DataSets for GSM2327014
Status Public on Sep 05, 2017
Title FAM13AKOAir [Sample033]
Sample type RNA
 
Source name mouse lung
Organism Mus musculus
Characteristics genotype: FAM13AKO
treatment: Air
strain: C57BL6
Treatment protocol Mice were exposed to mixed main-stream and side-stream CS from 3R4F Kentucky Research cigarettes for 5 days/week for 6 months in Teague TE 10z chambers (TSP ~ 100-200 mg/m 3 and CO levels ~6 ppm).
Extracted molecule total RNA
Extraction protocol RNA was extracted from murine lung tissues using Allprep kit (Qiagen, Valencia, CA).
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol 750ng of total RNA for each sample was hybridized onto a Sentrix MouseRef-8 v2.0 Expression BeadChip Array (Illumina, San Diego, CA), based on the manufacturer's protocol.
Scan protocol The BeadChip Array was scanned using an Illumina BeadArray Reader and quality control analysis was performed using Illumina GenomeStudio v3.1.3 software, Gene Expression Module version 1.1.1, and the statistical package R v3.3.1.
Description FAM13AKOAir [Sample033]
Data processing Each of the samples and all probes passed quality control, and the data were background corrected, log2 transformed and quantile normalized.
 
Submission date Sep 23, 2016
Last update date Sep 05, 2017
Contact name Jeong H Yun
E-mail(s) jeong.yun@channing.harvard.edu
Organization name BRIGHAM AND WOMEN'S HOSPITAL
Street address 181 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6885
Series (1)
GSE87292 Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways

Data table header descriptions
ID_REF
VALUE quantile normalized
Sample033.Detection.Pval

Data table
ID_REF VALUE Sample033.Detection.Pval
ILMN_1250052 7.343498988 0.00125
ILMN_3122480 7.058912901 0.23559
ILMN_2599935 8.219795907 0
ILMN_2675543 7.18491881 0.03133
ILMN_2686883 7.293852901 0.00627
ILMN_2751818 7.02880522 0.35338
ILMN_2728634 7.369573579 0
ILMN_3040515 7.175290094 0.03383
ILMN_2711608 7.175290094 0.03509
ILMN_1232875 7.212024542 0.0188
ILMN_1258507 7.22424103 0.01504
ILMN_2746142 7.076347962 0.18672
ILMN_1252690 7.305821391 0.00501
ILMN_2655499 6.986710554 0.58772
ILMN_1252870 7.129347221 0.08647
ILMN_1248179 7.014664381 0.41604
ILMN_2649955 7.638333595 0
ILMN_2628708 7.138846227 0.07143
ILMN_3024781 7.065834002 0.21303
ILMN_2705628 9.442588625 0

Total number of rows: 25697

Table truncated, full table size 758 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap