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Sample GSM2327021 Query DataSets for GSM2327021
Status Public on Sep 05, 2017
Title C57Smoke [Sample040]
Sample type RNA
 
Source name mouse lung
Organism Mus musculus
Characteristics genotype: WT
treatment: Smoke
strain: NZWLacJ
Treatment protocol Mice were exposed to mixed main-stream and side-stream CS from 3R4F Kentucky Research cigarettes for 5 days/week for 6 months in Teague TE 10z chambers (TSP ~ 100-200 mg/m 3 and CO levels ~6 ppm).
Extracted molecule total RNA
Extraction protocol RNA was extracted from murine lung tissues using Allprep kit (Qiagen, Valencia, CA).
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol 750ng of total RNA for each sample was hybridized onto a Sentrix MouseRef-8 v2.0 Expression BeadChip Array (Illumina, San Diego, CA), based on the manufacturer's protocol.
Scan protocol The BeadChip Array was scanned using an Illumina BeadArray Reader and quality control analysis was performed using Illumina GenomeStudio v3.1.3 software, Gene Expression Module version 1.1.1, and the statistical package R v3.3.1.
Description C57Smoke [Sample040]
Data processing Each of the samples and all probes passed quality control, and the data were background corrected, log2 transformed and quantile normalized.
 
Submission date Sep 23, 2016
Last update date Sep 05, 2017
Contact name Jeong H Yun
E-mail(s) jeong.yun@channing.harvard.edu
Organization name BRIGHAM AND WOMEN'S HOSPITAL
Street address 181 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6885
Series (1)
GSE87292 Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways

Data table header descriptions
ID_REF
VALUE quantile normalized
Sample040.Detection.Pval

Data table
ID_REF VALUE Sample040.Detection.Pval
ILMN_1250052 7.134097997 0.04261
ILMN_3122480 6.988400433 0.53885
ILMN_2599935 7.777222803 0
ILMN_2675543 6.95149026 0.7381
ILMN_2686883 7.446373488 0
ILMN_2751818 6.97720419 0.60401
ILMN_2728634 7.200801997 0.01128
ILMN_3040515 7.070943795 0.15038
ILMN_2711608 7.200262514 0.01128
ILMN_1232875 6.973013852 0.62782
ILMN_1258507 7.093905784 0.09649
ILMN_2746142 7.016741676 0.37469
ILMN_1252690 7.097781839 0.09023
ILMN_2655499 6.974441966 0.62281
ILMN_1252870 7.132975723 0.04386
ILMN_1248179 6.881090575 0.9787
ILMN_2649955 7.338004629 0
ILMN_2628708 7.115033429 0.07018
ILMN_3024781 6.974441966 0.62155
ILMN_2705628 8.039053366 0

Total number of rows: 25697

Table truncated, full table size 754 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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