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Sample GSM2327043 Query DataSets for GSM2327043
Status Public on Sep 05, 2017
Title FAM13AKOSmoke [Sample062]
Sample type RNA
 
Source name mouse lung
Organism Mus musculus
Characteristics genotype: FAM13AKO
treatment: Smoke
strain: C57BL6
Treatment protocol Mice were exposed to mixed main-stream and side-stream CS from 3R4F Kentucky Research cigarettes for 5 days/week for 6 months in Teague TE 10z chambers (TSP ~ 100-200 mg/m 3 and CO levels ~6 ppm).
Extracted molecule total RNA
Extraction protocol RNA was extracted from murine lung tissues using Allprep kit (Qiagen, Valencia, CA).
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol 750ng of total RNA for each sample was hybridized onto a Sentrix MouseRef-8 v2.0 Expression BeadChip Array (Illumina, San Diego, CA), based on the manufacturer's protocol.
Scan protocol The BeadChip Array was scanned using an Illumina BeadArray Reader and quality control analysis was performed using Illumina GenomeStudio v3.1.3 software, Gene Expression Module version 1.1.1, and the statistical package R v3.3.1.
Description FAM13AKOSmoke [Sample062]
Data processing Each of the samples and all probes passed quality control, and the data were background corrected, log2 transformed and quantile normalized.
 
Submission date Sep 23, 2016
Last update date Sep 05, 2017
Contact name Jeong H Yun
E-mail(s) jeong.yun@channing.harvard.edu
Organization name BRIGHAM AND WOMEN'S HOSPITAL
Street address 181 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6885
Series (1)
GSE87292 Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways

Data table header descriptions
ID_REF
VALUE quantile normalized
Sample062.Detection.Pval

Data table
ID_REF VALUE Sample062.Detection.Pval
ILMN_1250052 7.433593543 0.00125
ILMN_3122480 6.912272644 0.92732
ILMN_2599935 8.186086443 0
ILMN_2675543 7.05250152 0.25564
ILMN_2686883 7.345002294 0.00501
ILMN_2751818 7.039974422 0.29198
ILMN_2728634 7.252752385 0.01253
ILMN_3040515 7.26233298 0.00877
ILMN_2711608 7.273820474 0.00877
ILMN_1232875 7.229389395 0.01629
ILMN_1258507 7.047524883 0.2619
ILMN_2746142 6.877745478 0.98246
ILMN_1252690 7.153861429 0.04887
ILMN_2655499 7.048792371 0.26065
ILMN_1252870 7.033661189 0.3183
ILMN_1248179 7.101072845 0.11278
ILMN_2649955 7.611451797 0
ILMN_2628708 7.033661189 0.31579
ILMN_3024781 7.036165028 0.31078
ILMN_2705628 10.36737561 0

Total number of rows: 25697

Table truncated, full table size 765 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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