|
| Status |
Public on Sep 05, 2017 |
| Title |
C57Smoke [Sample099] |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
treatment: Smoke
strain: C57BL6
|
| Treatment protocol |
Mice were exposed to mixed main-stream and side-stream CS from 3R4F Kentucky Research cigarettes for 5 days/week for 6 months in Teague TE 10z chambers (TSP ~ 100-200 mg/m 3 and CO levels ~6 ppm).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from murine lung tissues using Allprep kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
750ng of total RNA for each sample was hybridized onto a Sentrix MouseRef-8 v2.0 Expression BeadChip Array (Illumina, San Diego, CA), based on the manufacturer's protocol.
|
| Scan protocol |
The BeadChip Array was scanned using an Illumina BeadArray Reader and quality control analysis was performed using Illumina GenomeStudio v3.1.3 software, Gene Expression Module version 1.1.1, and the statistical package R v3.3.1.
|
| Description |
C57Smoke [Sample099]
|
| Data processing |
Each of the samples and all probes passed quality control, and the data were background corrected, log2 transformed and quantile normalized.
|
| |
|
| Submission date |
Sep 23, 2016 |
| Last update date |
Sep 05, 2017 |
| Contact name |
Jeong H Yun |
| E-mail(s) |
jeong.yun@channing.harvard.edu
|
| Organization name |
BRIGHAM AND WOMEN'S HOSPITAL
|
| Street address |
181 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE87292 |
Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways |
|
