|
| Status |
Public on Sep 27, 2008 |
| Title |
Rest Of the Brain, Embryonic day 18.5, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
Strain: NMRI, Gender: N/A, Age: 18.5 embryonic days, Tissue:brain tissue without microvascular fragments
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to manufacturer's instructions (RNeasy Micro Kit (Qiagen, cat.# 74004) for embryonic tissue and RNeasy Mini Kit (Qiagen, cat.# 74104) for adult tissue)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix small sample amplification protocol from app. 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from rest brain at Embryonic day 18.5
|
| Data processing |
The data were analyzed with GCRMA package in the bioconductor project (R version 2.4.1, gcrma version 2.6.0).
|
| |
|
| Submission date |
Oct 01, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Liqun He |
| Organization name |
Karolinska Institute
|
| Street address |
Scheelesvag 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9202 |
Expression data from mouse microvascular transcriptomes |
|
| Relations |
| Reanalyzed by |
GSE119085 |
