|
Status |
Public on Oct 25, 2016 |
Title |
Gna11 ko ATII cell 2 |
Sample type |
RNA |
|
|
Source name |
Gna11 ko, d7 ATII cells
|
Organism |
Mus musculus |
Characteristics |
tissue: lung cell type: Type II alveolar epithelial cells genotype/variation: Gna11 ko
|
Treatment protocol |
Cell growth media was replace every 3 days
|
Growth protocol |
ATII cells were isolated from the lungs and grown for 7 days on collagen-coated tissue culture plates in Bronchial Epithelial Growth Medium (Lonza) without hydrocortisone and containing keratinocyte growth factor (10 μg/ml, R&D Systems)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
10 ng of total RNA was used to prepare microarray hybridization probes with Epistem’s RNAamp RNA amplification kits (Epistem, Manchester, UK)
|
|
|
Hybridization protocol |
Standard Affymetrix protocol (FS450_0001)
|
Scan protocol |
GCOS 4.00.1567
|
Description |
Gene expression data from d7 ATII cells from mice deficient in Gna11 13154_12xMAE_C02
|
Data processing |
RMA signal
|
|
|
Submission date |
Oct 11, 2016 |
Last update date |
Oct 25, 2016 |
Contact name |
Alison Elizabeth John |
E-mail(s) |
alison.john@nottingham.ac.uk
|
Phone |
01158231106
|
Organization name |
University of Nottingham
|
Department |
Respiratory Medicine
|
Street address |
Clinical Sciences Bulding, City Hospital Campus
|
City |
Nottingham |
State/province |
NOTTS |
ZIP/Postal code |
NG5 1PB |
Country |
United Kingdom |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE87842 |
Role of Gq/11 and G12/13 signalling in Type II alveolar epithelial cells |
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