|
| Status |
Public on Oct 25, 2016 |
| Title |
Gna12/Ga13 ko ATII cell 3 |
| Sample type |
RNA |
| |
|
| Source name |
Gna12/Ga13 ko, d7 ATII cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
cell type: Type II alveolar epithelial cells
genotype/variation: Gna12/Ga13 ko
|
| Treatment protocol |
Cell growth media was replace every 3 days
|
| Growth protocol |
ATII cells were isolated from the lungs and grown for 7 days on collagen-coated tissue culture plates in Bronchial Epithelial Growth Medium (Lonza) without hydrocortisone and containing keratinocyte growth factor (10 μg/ml, R&D Systems)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
10 ng of total RNA was used to prepare microarray hybridization probes with Epistem’s RNAamp RNA amplification kits (Epistem, Manchester, UK)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (FS450_0001)
|
| Scan protocol |
GCOS 4.00.1567
|
| Description |
Gene expression data from d7 ATII cells from mice deficient in Gna12 and Gna13
13154_12xMAE_G04
|
| Data processing |
RMA signal
|
| |
|
| Submission date |
Oct 11, 2016 |
| Last update date |
Oct 25, 2016 |
| Contact name |
Alison Elizabeth John |
| E-mail(s) |
alison.john@nottingham.ac.uk
|
| Phone |
01158231106
|
| Organization name |
University of Nottingham
|
| Department |
Respiratory Medicine
|
| Street address |
Clinical Sciences Bulding, City Hospital Campus
|
| City |
Nottingham |
| State/province |
NOTTS |
| ZIP/Postal code |
NG5 1PB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE87842 |
Role of Gq/11 and G12/13 signalling in Type II alveolar epithelial cells |
|
