|
Status |
Public on Mar 30, 2008 |
Title |
slr-2(ku297) worms at L1 stage, third replicate |
Sample type |
RNA |
|
|
Source name |
RNA from larval C. elegans population
|
Organism |
Caenorhabditis elegans |
Characteristics |
slr-2(ku297) L1 larvae
|
Biomaterial provider |
David Fay
|
Growth protocol |
Worms were grown on NGM plates spotted with OP50 at 20 oC.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with phenol, cleaned with phenol:chlorophorm:isoamyl alcohol (25:24:1), and then with chlorophorm:isoamyl alcohol (24:1). Total RNA was precipitated overnight at 4 oC with 4M lithium acetate and DNAseI treated before purification on RNEasy minicolumns from Qiagen.
|
Label |
biotin
|
Label protocol |
2.5 ug total RNA were used for cDNA synthesis. cDNA synthesis, cRNA synthesis, fragmentation and labeling were performed according to Affymetrix protocol.
|
|
|
Hybridization protocol |
according to Affymetrix protocol
|
Scan protocol |
according to Affymetrix protocol
|
Description |
slr-2(ku297) larvae
|
Data processing |
expression values were generated using RMA
|
|
|
Submission date |
Oct 05, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
David Fay |
E-mail(s) |
davidfay@uwyo.edu
|
Organization name |
University of Wyoming
|
Department |
MOLB
|
Street address |
1000 E. University Dept. 3944
|
City |
Laramie |
State/province |
WY |
ZIP/Postal code |
82071 |
Country |
USA |
|
|
Platform ID |
GPL200 |
Series (1) |
GSE9246 |
Transcriptome profiling of slr-2, C.elegans C2H2 Zn-finger |
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