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Sample GSM234791 Query DataSets for GSM234791
Status Public on Mar 30, 2008
Title slr-2(ku297) worms at L1 stage, third replicate
Sample type RNA
 
Source name RNA from larval C. elegans population
Organism Caenorhabditis elegans
Characteristics slr-2(ku297) L1 larvae
Biomaterial provider David Fay
Growth protocol Worms were grown on NGM plates spotted with OP50 at 20 oC.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with phenol, cleaned with phenol:chlorophorm:isoamyl alcohol (25:24:1), and then with chlorophorm:isoamyl alcohol (24:1). Total RNA was precipitated overnight at 4 oC with 4M lithium acetate and DNAseI treated before purification on RNEasy minicolumns from Qiagen.
Label biotin
Label protocol 2.5 ug total RNA were used for cDNA synthesis. cDNA synthesis, cRNA synthesis, fragmentation and labeling were performed according to Affymetrix protocol.
 
Hybridization protocol according to Affymetrix protocol
Scan protocol according to Affymetrix protocol
Description slr-2(ku297) larvae
Data processing expression values were generated using RMA
 
Submission date Oct 05, 2007
Last update date Aug 14, 2011
Contact name David Fay
E-mail(s) davidfay@uwyo.edu
Organization name University of Wyoming
Department MOLB
Street address 1000 E. University Dept. 3944
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL200
Series (1)
GSE9246 Transcriptome profiling of slr-2, C.elegans C2H2 Zn-finger

Data table header descriptions
ID_Ref
VALUE RMA generated expression values, log(2)

Data table
ID_Ref VALUE
171720_x_at 7.51330453
171721_x_at 8.625974139
171722_x_at 10.15669567
171723_x_at 5.174255757
171724_x_at 5.851348204
171725_x_at 12.58223336
171726_x_at 6.234827909
171727_x_at 5.464966968
171728_x_at 8.167563422
171729_x_at 9.952256639
171730_x_at 8.943547492
171731_x_at 9.27663064
171732_x_at 8.153784476
171733_x_at 6.053159983
171734_x_at 12.69605837
171735_x_at 12.15523478
171736_x_at 7.388660989
171737_x_at 10.86745916
171738_x_at 10.43655706
171739_x_at 9.687264752

Total number of rows: 22625

Table truncated, full table size 497 Kbytes.




Supplementary file Size Download File type/resource
GSM234791.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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