|
| Status |
Public on Nov 01, 2007 |
| Title |
Lymphoma lambda MYCtg/AIDKO 4 |
| Sample type |
RNA |
| |
|
| Source name |
nodal B-NHL from lambda MYC transgenic/AIDKO mouse 4
|
| Organism |
Mus musculus |
| Characteristics |
Strain: mixed C57BL/6xSv129
Tissue: frozen biopsy of lymph node tumor
|
| Treatment protocol |
Nodal tumors were isolated,frozen in dry ice and stored at -80C.
|
| Growth protocol |
Anymals were monitored biweekly and sacrificed upon evidence of tumor formation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for at least 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from nodal B-NHL of lMYC transgenic/AIDKO mouse
|
| Data processing |
The data were analyzed with MAS5 in the Affymetrix GeneChip Operating Software (GCOS v1.2) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Oct 05, 2007 |
| Last update date |
Jan 10, 2023 |
| Contact name |
Laura Pasqualucci |
| E-mail(s) |
lp171@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1130 St Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9249 |
Gene expression analysis of B-NHL from λMYC, λMYC/IµHABCL6, λMYC/AIDKO and λMYC/IµHABCL6/AIDKO mouse models |
|
| Relations |
| Reanalyzed by |
GSE119085 |
