|
Status |
Public on Feb 28, 2008 |
Title |
High-calcium diet, rep2 |
Sample type |
RNA |
|
|
Source name |
dietary calcium and dairy proteins, sample 8815
|
Organism |
Mus musculus |
Characteristics |
9-week-old C57Bl/6J-mice were divided into two groups (n=10/group). The control diet was a standard high-fat diet (60% of energy) low in calcium (0.4%). The whey protein diet was a high-calcium (1.8%) high-fat diet with whey protein isolate. After the 21-week treatment, the adipose tissue transcript profiling (2 mice/group) was carried out using Affymetrix Mouse Genome 430 2.0 array.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from the epididymal fat pads of two control mice, and two high-calcium whey protein fed mice were collected with Trizol (Gibco), purified with RNeasy Mini Kit (Qiagen) and measured with 260 and 280 nm.
|
Label |
biotin
|
Label protocol |
according the standard protocol
|
|
|
Hybridization protocol |
according the standard protocol
|
Scan protocol |
according the standard protocol
|
Description |
9-week-old C57Bl/6J-mice were divided into two groups (n=10/group). The control diet was a standard high-fat diet (60% of energy) low in calcium (0.4%). The whey protein diet was a high-calcium (1.8%) high-fat diet with whey protein isolate. After the 21-week treatment, the adipose tissue transcript profiling (2 mice/group) was carried out using Affymetrix Mouse Genome 430 2.0 array.
|
Data processing |
The data was pre-processed with the robust multichip algorithm (RMA), normalized per chip to the median, and analyzed with Genespring 7.2. (Agilent, USA).
|
|
|
Submission date |
Oct 10, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Markus Storvik |
Organization name |
University of Eastern Finland
|
Department |
Pharmacology
|
Street address |
University of Eastern Finland
|
City |
Kuopio |
ZIP/Postal code |
70211 |
Country |
Finland |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9280 |
The effect of dietary calcium and dairy proteins on adipose tissue gene expression profile in diet induced obesity |
|
Relations |
Reanalyzed by |
GSE119085 |