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Sample GSM235533 Query DataSets for GSM235533
Status Public on Feb 28, 2008
Title High-calcium diet, rep2
Sample type RNA
 
Source name dietary calcium and dairy proteins, sample 8815
Organism Mus musculus
Characteristics 9-week-old C57Bl/6J-mice were divided into two groups (n=10/group). The control diet was a standard high-fat diet (60% of energy) low in calcium (0.4%). The whey protein diet was a high-calcium (1.8%) high-fat diet with whey protein isolate. After the 21-week treatment, the adipose tissue transcript profiling (2 mice/group) was carried out using Affymetrix Mouse Genome 430 2.0 array.
Extracted molecule total RNA
Extraction protocol Total RNA from the epididymal fat pads of two control mice, and two high-calcium whey protein fed mice were collected with Trizol (Gibco), purified with RNeasy Mini Kit (Qiagen) and measured with 260 and 280 nm.
Label biotin
Label protocol according the standard protocol
 
Hybridization protocol according the standard protocol
Scan protocol according the standard protocol
Description 9-week-old C57Bl/6J-mice were divided into two groups (n=10/group). The control diet was a standard high-fat diet (60% of energy) low in calcium (0.4%). The whey protein diet was a high-calcium (1.8%) high-fat diet with whey protein isolate. After the 21-week treatment, the adipose tissue transcript profiling (2 mice/group) was carried out using Affymetrix Mouse Genome 430 2.0 array.
Data processing The data was pre-processed with the robust multichip algorithm (RMA), normalized per chip to the median, and analyzed with Genespring 7.2. (Agilent, USA).
 
Submission date Oct 10, 2007
Last update date Aug 28, 2018
Contact name Markus Storvik
Organization name University of Eastern Finland
Department Pharmacology
Street address University of Eastern Finland
City Kuopio
ZIP/Postal code 70211
Country Finland
 
Platform ID GPL1261
Series (1)
GSE9280 The effect of dietary calcium and dairy proteins on adipose tissue gene expression profile in diet induced obesity
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Processed expression value
FLAG P=present, M=marginal, A=absent

Data table
ID_REF VALUE FLAG
1418185_at 0.864 P
1428669_at 0.236955927272727 P
1437819_s_at 0.0883479272727273 M
1420295_x_at 0.945687272727273 P
1427917_s_at 0.417610472727273 P
1454851_at 0.909870545454545 P
1453284_at 0.226587927272727 P
1442332_at 0.0894161454545455 A
1452816_at 0.0172171636363636 A
1451249_at 0.3131136 P
1422773_at 0.0290932363636364 A
1439850_at 0.0674862545454546 A
1427331_at 0.136103563636364 A
1420738_at 0.00466874181818182 A
1438283_at 0.0789853090909091 P
1437815_at 0.00538507636363636 A
1436168_at 2.13706472727273 P
1428335_a_at 2.674944 P
1430272_at 0.0865256727272727 A
1440756_at 0.0111220363636364 A

Total number of rows: 45101

Table truncated, full table size 1377 Kbytes.




Supplementary file Size Download File type/resource
GSM235533.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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