NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM235592 Query DataSets for GSM235592
Status Public on Nov 27, 2007
Title Day 22-35 Chronic infection GP33 specific-2_MG-U74C
Sample type RNA
 
Source name Mouse Spleen CD8 T cells
Organism Mus musculus
Characteristics C57Bl/6
Day 22-35 Chronic LCMV infection
Treatment protocol Virus specific CD8 T cells were sorted by flow cytometry and purified cells were placed in Trizol Solution (GibcoBRL)
Growth protocol T cells were isolated from naïve 4-6 week old mice on day 22-35 of chronic LCMV infection
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix hybridization protocols for eukaryotic total RNA were used for the samples.All samples were hybed at 45C16 hours60 RPM in 640 Affymetrix Hyb oven. Affymetrix fludics program EUKGEWS2v4_450 was used for washing
Scan protocol GeneChips were scanned using the Affymetrix 3000 7G Plus
Description The array used was Murine Genome U74 Version 2 Set MG-U74C. There are 4 groups Naïve Effector Memory and Exhausted . Each group has 3 biological replicates. The biological replicates are numbered 1 to 4; except Effectors 1 to 3 .The
This sample is called Exhausted-2
Data processing Genespring
 
Submission date Oct 10, 2007
Last update date Aug 14, 2011
Contact name Shruti Subramaniam
Organization name Emory University
Street address Clifton Road
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platform ID GPL83
Series (1)
GSE9650 Chronic viral infection of naive, effector, memory and exhausted virus-specific CD8 T cells

Data table header descriptions
ID_REF ProbesetID
VALUE Genespring calculated the Signal values

Data table
ID_REF VALUE
128520_at 629.7
128523_at 7.295
128529_at 50.69
128550_at 199.9
128554_at 19.36
128577_s_at 22.43
128578_r_at 60.01
128579_r_at 105.4
128580_at 49.8
128582_at 15.28
128583_at 8.832
128590_at 37.95
128624_at 99.64
128643_r_at 110.8
128644_at 138.1
128645_at 99.16
128646_at 55.22
128647_at 36.15
128651_at 163.5
128652_at 27.03

Total number of rows: 11868

Table truncated, full table size 192 Kbytes.




Supplementary file Size Download File type/resource
GSM235592.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap