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Status |
Public on Mar 08, 2017 |
Title |
NAc_saline_7d |
Sample type |
RNA |
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Source name |
Nucleus accumbens
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Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley gender: male age: 8-10 weeks tissue: brain tissue compartment: Nucleus accumbens treatment: Saline
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Treatment protocol |
Rats were treated with saline or heroin (1 mg/kg, i.p., b.i.d.) for 7 days. The nucleus accumbens of three rats from each group were mixed and homogenized in TRIzol.
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Growth protocol |
Six-week-old Male Sprague-Dawley rats were purchased from the Shanghai SLAC Laboratory Animal Co. Ltd. and housed with a 12 h reverse dark/light cycle at 23℃. All subjects were allowed free access to food and water. 8-10 weeks old rats were used for experiments.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA are harvested using TRIzol (Invitrogen) and RNeasy mini kit (QIAGEN) according to manufacturer’s instructions.
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Label |
Hy3
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Label protocol |
The samples are labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit.
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Hybridization protocol |
The samples were hybridized on a hybridization station. Array = miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno. The miRNA associated with the probe ID is available in the .gal file on the series record.
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Scan protocol |
Scanning is performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 is used to read the raw intensity of the image.
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Description |
Sample name: S Rats were treated with heroin (1 mg/kg, i.p., b.i.d.) for 7 days. The nucleus accumbens of three rats were mixed and homogenized in TRIzol.
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Data processing |
The intensity of green signal is calculated after background subtraction and replicated spots on the same slide have been averaged by getting a median intensity. We use Median Normalization Method to obtain “Normalized Data”, Normalized Data=(Foreground-Background)/median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction. The processed data file includes probe ID and median-normalized signal.
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Submission date |
Oct 26, 2016 |
Last update date |
Mar 08, 2017 |
Contact name |
Qiumin Le |
E-mail(s) |
qle09@fudan.edu.cn
|
Organization name |
Fudan University
|
Street address |
130 Dong'an Road
|
City |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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|
Platform ID |
GPL17107 |
Series (1) |
GSE89202 |
MiR-218 regulates heroin seeking behavior by targeting Mecp2 |
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