|
| Status |
Public on Dec 15, 2009 |
| Title |
spleen_Yaa_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
spleen cells from C57Bl/6 male with Yaa mutation
|
| Organism |
Mus musculus |
| Characteristics |
Strain: B6.SB-Yaa/J
Sex: male
Tissue: whole spleen tissue
|
| Biomaterial provider |
Jackson Labs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Combination of Trizol protocol followed by Qiagen RNeasy column
|
| Label |
biotin
|
| Label protocol |
Affymetrix One cycle target labeling kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization plus EukGE-WS2v5 fluidics protocol
|
| Scan protocol |
Affymetrix 7G scanner
|
| Description |
The Yaa gene is on the Y chromosome and is responsible for accelerated autoimmune disease in mice with susceptible genetic backgrounds
|
| Data processing |
Affymetrix MAS5.0 normalization & transforming algorithm
|
| |
|
| Submission date |
Oct 10, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Bart Frank |
| E-mail(s) |
frank@omrf.org
|
| Organization name |
OMRF
|
| Street address |
825 NE 13 St
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73103 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9287 |
Differential gene expression in wild-type versus Yaa mutant mouse spleen cells |
|
