|
| Status |
Public on Oct 12, 2007 |
| Title |
Ovary postnatal day 4 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
P4-day rat ovary
|
| Organism |
Rattus norvegicus |
| Characteristics |
ovaries dissected from P4-day old Sprague-Dawley rats and cleaned of ovarian bursa.
|
| Treatment protocol |
Female day P4 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure
|
| Scan protocol |
chips were scanned using Agilent GeneArray Scanner G2500A
|
| Description |
Chip ID=Phil-P4-RGu34A-072403. RNA from four-day old rat ovaries.
|
| Data processing |
Auto-scale ON (1-1000). Scaling = all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
|
| |
|
| Submission date |
Oct 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Michael K Skinner |
| E-mail(s) |
skinner@mail.wsu.edu
|
| Organization name |
WSU
|
| Department |
SBS
|
| Street address |
Abelson 507
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99163 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE9300 |
Alterations in the ovarian transcriptome during primordial follicle assembly and development. |
|
