|
| Status |
Public on Sep 01, 2008 |
| Title |
ATF2 WT-1 |
| Sample type |
RNA |
| |
|
| Source name |
RNA was prepared from papilomas of 2-3 mice (2 for WT and 3 for Knockout) and this was pooled to perform the array. Each pooled sample was run in triplicates. The mice were treated once with DMBA, followed by TPA application for 30 wks.
|
| Organism |
Mus musculus |
| Characteristics |
ATF2 Genotype: WT
|
| Treatment protocol |
The mice were treated once with DMBA, followed by TPA application for 30 wks.
|
| Growth protocol |
These mice are mixed background of FVB and C57bl6 and the age when they got the papillomas was about 9 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared from papilomas of 2-3 mice (2 for WT and 3 for Knockout) and this was pooled to perform the array.
|
| Label |
Cy3
|
| Label protocol |
Labeling was per Illumina standard protocol.
|
| |
|
| Hybridization protocol |
Hybridization was per Illumina standard protocol
|
| Scan protocol |
Images were scanned on an Illumina BeadStation scanner
|
| Description |
Technical replicate #1 of ATF2 wildtype. These mice are mixed background of FVB and C57bl6 and the age when they got the papillomas was about 9 months.
|
| Data processing |
Data were extracted using BeadStudio version 3 without any normalization and were then processed after log2 transformation using quantile normalization.
|
| |
|
| Submission date |
Oct 15, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sean Davis |
| E-mail(s) |
sdavis2@mail.nih.gov
|
| Phone |
301-435-2652
|
| Organization name |
National Cancer Institute
|
| Lab |
Genetics Branch
|
| Street address |
37 Convent Drive, Room 6138
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE9328 |
ATF2 knockout in papillomas |
|
