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Sample GSM237748 Query DataSets for GSM237748
Status Public on Jan 19, 2008
Title domesticus male 2, biological rep2, brain
Sample type RNA
 
Source name brain
Organism Mus musculus domesticus
Characteristics Gender: male, age: 45 days, tissue: brain
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the Ambion MessageAmp™ II aRNA Amplification Kit from 1ug total RNA.
 
Hybridization protocol Following fragmentation, 12ug of cRNA were hybridized for 16-18 hr at 45 °C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000 scanner.
Description Gene expression data from M. m. domesticus brain
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Bioconductor default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 660 for brain and testis and to 800 for liver.
 
Submission date Oct 16, 2007
Last update date Aug 28, 2018
Contact name Ruth Rottscheidt
E-mail(s) ruth.rottscheidt@uni-koeln.de
Organization name University of Cologne
Department Institute for Genetics
Lab Tautz Harr Mouse Evolutionary Genomics
Street address Zuelpicher Strasse 47
City Cologne
ZIP/Postal code 50674
Country Germany
 
Platform ID GPL1261
Series (1)
GSE9338 Expression data from Mus musculus subspecies and their F1 hybrids for 3 tissues (brain, liver, testis)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1269
1415671_at 6894
1415672_at 5767
1415673_at 925
1415674_a_at 2027
1415675_at 1495
1415676_a_at 4796
1415677_at 1182
1415678_at 3269
1415679_at 4984
1415680_at 375
1415681_at 1661
1415682_at 845
1415683_at 1911
1415684_at 762
1415685_at 1045
1415686_at 3107
1415687_a_at 12367
1415688_at 2749
1415689_s_at 1189

Total number of rows: 45037

Table truncated, full table size 662 Kbytes.




Supplementary file Size Download File type/resource
GSM237748.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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