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Sample GSM237757 Query DataSets for GSM237757
Status Public on Jan 19, 2008
Title F1 hybrid (musculus mother x castaneus father) 1, biological rep1, brain
Sample type RNA
 
Source name brain
Organism Mus musculus musculus x M. m. castaneus
Characteristics Gender: male, age: 53 days, tissue: brain
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the Ambion MessageAmp™ II aRNA Amplification Kit from 1ug total RNA.
 
Hybridization protocol Following fragmentation, 12ug of cRNA were hybridized for 16-18 hr at 45 °C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000 scanner.
Description Gene expression data from M. m. musculus mother x M. m. castaneus father F1 hybrids, brain
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Bioconductor default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 660 for brain and testis and to 800 for liver.
 
Submission date Oct 16, 2007
Last update date Aug 28, 2018
Contact name Ruth Rottscheidt
E-mail(s) ruth.rottscheidt@uni-koeln.de
Organization name University of Cologne
Department Institute for Genetics
Lab Tautz Harr Mouse Evolutionary Genomics
Street address Zuelpicher Strasse 47
City Cologne
ZIP/Postal code 50674
Country Germany
 
Platform ID GPL1261
Series (1)
GSE9338 Expression data from Mus musculus subspecies and their F1 hybrids for 3 tissues (brain, liver, testis)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1620
1415671_at 5813
1415672_at 5294
1415673_at 620
1415674_a_at 1489
1415675_at 1186
1415676_a_at 3373
1415677_at 829
1415678_at 2726
1415679_at 3335
1415680_at 366
1415681_at 1280
1415682_at 983
1415683_at 2347
1415684_at 497
1415685_at 1259
1415686_at 3187
1415687_a_at 10291
1415688_at 2552
1415689_s_at 916

Total number of rows: 45037

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM237757.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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