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Sample GSM237780 Query DataSets for GSM237780
Status Public on Jan 19, 2008
Title F1 hybrid (musculus mother x domesticus father) 2, biological rep2, testis
Sample type RNA
Source name testis
Organism Mus musculus musculus x M. m. domesticus
Characteristics Gender: male, age: 53 days, tissue: testis
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the Ambion MessageAmp™ II aRNA Amplification Kit from 1ug total RNA.
Hybridization protocol Following fragmentation, 12ug of cRNA were hybridized for 16-18 hr at 45 °C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000 scanner.
Description Gene expression data from M. m. musculus mother x M. m. domesticus father F1 hybrids, testis
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Bioconductor default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 660 for brain and testis and to 800 for liver.
Submission date Oct 16, 2007
Last update date Aug 28, 2018
Contact name Ruth Rottscheidt
Organization name University of Cologne
Department Institute for Genetics
Lab Tautz Harr Mouse Evolutionary Genomics
Street address Zuelpicher Strasse 47
City Cologne
ZIP/Postal code 50674
Country Germany
Platform ID GPL1261
Series (1)
GSE9338 Expression data from Mus musculus subspecies and their F1 hybrids for 3 tissues (brain, liver, testis)
Reanalyzed by GSE119085

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
1415670_at 1078
1415671_at 1758
1415672_at 3732
1415673_at 625
1415674_a_at 1912
1415675_at 991
1415676_a_at 3000
1415677_at 2614
1415678_at 3343
1415679_at 5330
1415680_at 690
1415681_at 1332
1415682_at 509
1415683_at 3798
1415684_at 251
1415685_at 860
1415686_at 717
1415687_a_at 4015
1415688_at 1021
1415689_s_at 505

Total number of rows: 45037

Table truncated, full table size 661 Kbytes.

Supplementary file Size Download File type/resource
GSM237780.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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