|
Status |
Public on Dec 14, 2007 |
Title |
mammary tumor CR115-4, transplanted, unsorted |
Sample type |
RNA |
|
|
Source name |
mammary tumor isolated from a RAG2-/- recipient #4 transplanted with primary tumor from mouse CR115, unsorted
|
Organism |
Mus musculus |
Characteristics |
Wap-Cre;Etv6-NTRK3 parous female
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
|
Scan protocol |
Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
|
Description |
Gene expression data from unfractionated mammary tumor
|
Data processing |
The raw CEL files were imported into dChip and normalized with dChip.
|
|
|
Submission date |
Oct 17, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Zhe Li |
E-mail(s) |
li@bloodgroup.tch.harvard.edu
|
Phone |
617-919-2052
|
Organization name |
Children's Hospital Boston
|
Department |
Division of Hematology/Oncology
|
Lab |
Stuart Orkin's Lab
|
Street address |
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9355 |
Microarray expression profiles of mammary tumors developed in Wap-Cre;Etv6-NTRK3 mice |
|
Relations |
Reanalyzed by |
GSE119085 |