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Sample GSM238366 Query DataSets for GSM238366
Status Public on Dec 14, 2007
Title hyperplastic MECs G376H, sorted
Sample type RNA
 
Source name hyperplastic mammary epithelial cells from mouse G376, sorted
Organism Mus musculus
Characteristics Wap-Cre;Etv6-NTRK3;Rosa-Stop-lacZ nulliparous female
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Description Gene expression data from sorted hyperplastic mammary epithelial cells
Data processing The raw CEL files were imported into dChip and normalized with dChip.
 
Submission date Oct 17, 2007
Last update date Aug 28, 2018
Contact name Zhe Li
E-mail(s) li@bloodgroup.tch.harvard.edu
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE9355 Microarray expression profiles of mammary tumors developed in Wap-Cre;Etv6-NTRK3 mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE After normalization, the model-based expression values were calculated by dChip and exported.
G376H call
G376H SE

Data table
ID_REF VALUE G376H call G376H SE
AFFX-BioB-5_at 4373.53 P 257.002
AFFX-BioB-M_at 5232.65 P 310.05
AFFX-BioB-3_at 4166.81 P 456.138
AFFX-BioC-5_at 7449.95 P 524.194
AFFX-BioC-3_at 6195.6 P 494.986
AFFX-BioDn-5_at 8236.48 P 1098.49
AFFX-BioDn-3_at 8291.43 P 1115.73
AFFX-CreX-5_at 11168.3 P 1444.82
AFFX-CreX-3_at 10797.1 P 1190.69
AFFX-DapX-5_at 11.893 A 3.96137
AFFX-DapX-M_at 4.3207 A 5.04721
AFFX-DapX-3_at 11.7322 A 3.35259
AFFX-LysX-5_at 11.6426 P 6.77009
AFFX-LysX-M_at 3.0967 A 4.34896
AFFX-LysX-3_at 49.0435 A 4.58227
AFFX-PheX-5_at 15.9428 A 5.58591
AFFX-PheX-M_at 18.4823 A 5.60557
AFFX-PheX-3_at 45.6769 A 12.7084
AFFX-ThrX-5_at 8.2581 A 3.94425
AFFX-ThrX-M_at 27.2189 A 6.16444

Total number of rows: 45101

Table truncated, full table size 1284 Kbytes.




Supplementary file Size Download File type/resource
GSM238366.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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