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Sample GSM238463 Query DataSets for GSM238463
Status Public on Nov 01, 2007
Title rhPBEF rep1 (age 8-12 weeks)
Sample type RNA
 
Source name Lung
Organism Mus musculus
Characteristics C57BL/6J
22-32g
Age: 8-12 week
Biomaterial provider Charles Rivers Laboratories (Wilmington, MA)
Treatment protocol rhPBEF 20µg / mouse in 1.5µl water per gram body weight, intratracheal injection
Growth protocol standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from lung tissue of control animal
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization;
 
Submission date Oct 18, 2007
Last update date Aug 28, 2018
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL1261
Series (1)
GSE9368 Mouse lung with recombinant human soluble PBEFtreatment and ventilator-induced lung injury: age 8-12wks
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1415670_at 9.947885671 P
1415671_at 11.33529712 P
1415672_at 11.89189843 P
1415673_at 7.760460222 P
1415674_a_at 9.505709143 P
1415675_at 9.386254403 P
1415676_a_at 11.49110048 P
1415677_at 9.621093668 P
1415678_at 11.33675506 P
1415679_at 11.65464811 A
1415680_at 9.658592514 A
1415681_at 10.2553313 A
1415682_at 8.436651462 A
1415683_at 11.01699823 A
1415684_at 8.768946018 P
1415685_at 8.786286068 A
1415686_at 10.30312228 A
1415687_a_at 13.14614393 A
1415688_at 10.81171443 A
1415689_s_at 9.945633996 A

Total number of rows: 45101

Table truncated, full table size 1114 Kbytes.




Supplementary file Size Download File type/resource
GSM238463.CEL.gz 3.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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