|
| Status |
Public on Oct 10, 2009 |
| Title |
Hypoxia-2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse quadriceps(QF) muscle
|
| Organism |
Mus musculus |
| Characteristics |
Adult male C57Bl/10 mouse
|
| Treatment protocol |
Adult male C57Bl/10 mice were used in experiments. At the beginning of the experiment mice were divided into two groups, control (room condition) and hypoxic (hypoxic condition), over a period of 2 weeks. The hypoxic group was gradually exposed to lower levels of hypoxia in an especially designed and hermetically closed hypoxic chamber. A Pegas 4000 MF(Columbus Instruments) gas blending system was used. The oxygen level was gradually decreased from 21% to 8% over one week and animals were kept at 8% oxygen for another 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each QF muscle by Tri-Reagent (Ambion, Austin, TX) as described by manufacturer. Isolated total RNA were cleaned up by RNeasy mini kit (Qiagen, Valencia, CA) as described by manufacturer.
|
| Label |
Biotin labeled
|
| Label protocol |
Please see GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA) protocol.
|
| |
|
| Hybridization protocol |
Please see GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA) protocol.
|
| Scan protocol |
Agilent model G2500A GeneArray scanner used.
|
| Description |
The purity and concentration of total RNA were determined by measurement of absorbance at 260 and 280nm using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). To satisfy our purity criteria, we discarded all RNA samples that did not have a 260/280 ratio between 1.8 and 2.1. To satisfy our criteria for integrity, we required that all RNA used in our experiments have single peaks for the 18S and 28S bands as determined by the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA).
|
| Data processing |
MAS5 normalized data
|
| |
|
| Submission date |
Oct 22, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Murat T Budak |
| E-mail(s) |
mtbudak@mail.med.upenn.edu
|
| Phone |
215-8989726
|
| Organization name |
University of Pennsylvania
|
| Department |
|
| Lab |
Rubinstein and Khurana Labs
|
| Street address |
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9400 |
Hypoxic gene regulation on mice Quadriceps muscle |
|
