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Status |
Public on Sep 13, 2010 |
Title |
a849sh_136914 |
Sample type |
RNA |
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Source name |
murine splenocytes
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Organism |
Mus musculus |
Characteristics |
C57BL/6J 8 weeks
|
Treatment protocol |
Splenocyte isolation from macerated spleen followed by RBC lysis with proprietary buffer, followed by a wash in Dulbeco's PBS followed by RLT suspension process.
|
Extracted molecule |
total RNA |
Extraction protocol |
Isolation of RNA was then performed using the Qiagen (Valencia, CA) recommendated protocol for the RNeasy method with DNase treatment.
|
Label |
biotin
|
Label protocol |
20 ng of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions. This was compared with using 1-2 ug of total RNA following the Affymetrix standard protocol.
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|
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Hybridization protocol |
labeled targets were hybridized to Affymetrix(Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
|
Scan protocol |
Scanned in an Affymetrix scanner according to Affymetrix standard protocols.
|
Description |
Sham
|
Data processing |
Gene expression values were ex-tracted and normalized for all 24 chips simultaneously using Dchip.
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|
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Submission date |
Oct 24, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Wenzhong Xiao, Inflammation & the Host Response to Injury |
E-mail(s) |
data_curator@gluegrant.org
|
Phone |
617 726 0082
|
Organization name |
Massachusetts General Hospital
|
Department |
Department of Surgery
|
Lab |
Burn & Trauma Research
|
Street address |
55 Fruit Street, GRV 1302
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9424 |
Assessing Statistical Significance in Microarray Experiments Using the Distance Between Microarrays |
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