|
| Status |
Public on Sep 13, 2010 |
| Title |
a851tr_136916 |
| Sample type |
RNA |
| |
|
| Source name |
murine splenocytes
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6J 8 weeks
|
| Treatment protocol |
Splenocyte isolation from macerated spleen followed by RBC lysis with proprietary buffer, followed by a wash in Dulbeco's PBS followed by RLT suspension process.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was then performed using the Qiagen (Valencia, CA) recommendated protocol for the RNeasy method with DNase treatment.
|
| Label |
biotin
|
| Label protocol |
20 ng of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions. This was compared with using 1-2 ug of total RNA following the Affymetrix standard protocol.
|
| |
|
| Hybridization protocol |
labeled targets were hybridized to Affymetrix(Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
|
| Scan protocol |
Scanned in an Affymetrix scanner according to Affymetrix standard protocols.
|
| Description |
Trauma/Hemorrhage
|
| Data processing |
Gene expression values were ex-tracted and normalized for all 24 chips simultaneously using Dchip.
|
| |
|
| Submission date |
Oct 24, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Wenzhong Xiao, Inflammation & the Host Response to Injury |
| E-mail(s) |
data_curator@gluegrant.org
|
| Phone |
617 726 0082
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Department of Surgery
|
| Lab |
Burn & Trauma Research
|
| Street address |
55 Fruit Street, GRV 1302
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9424 |
Assessing Statistical Significance in Microarray Experiments Using the Distance Between Microarrays |
|
