|
| Status |
Public on Nov 20, 2007 |
| Title |
HGF, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Madin Darby canine kidney (MDCK) cells, exposed to HGF
|
| Organism |
Canis lupus familiaris |
| Characteristics |
MDCK type II cells
|
| Treatment protocol |
MDCK cells grown on a Transwell filter for 7 days exposed to 24 hours of hepatocyte growth factor at 100 ng/ml
|
| Growth protocol |
MDCK cells grown on a Transwell filter in a 37 °C 5% CO2 incubator and fed MEM + 5% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen extraction of total RNA according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on Canine Genome 2.0 Array (Affymetrix).
|
| Scan protocol |
GeneChips were scanned at 3-nm resolution after excitation at 570 nm.
|
| Description |
Gene expression data from MDCK cells grown on a Transwell filter and exposed to HGF
|
| Data processing |
GCRMA-calculated Signal Intensity and Affymetrix flags using ArrayAssist Lite v3.4, Stratagene
|
| |
|
| Submission date |
Oct 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Xiaofeng Zuo |
| Organization name |
University of Pennsylvania
|
| Lab |
Josh Lipschutz
|
| Street address |
380 S. University Ave.
|
| City |
philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE9435 |
Expression data from MDCK cells exposed to HGF +/- MAPK inhibitors |
|
