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Sample GSM239853 Query DataSets for GSM239853
Status Public on Dec 07, 2007
Title L_AK_SleepDep_ZT 6_1
Sample type RNA
 
Source name AKR/J, sleep deprived, time of sacrifice ZT 6, replicat 1, Liver
Organism Mus musculus
Characteristics Strain: AKR/J, Gender: male, Age: 12-13 weeks of age, Tissue: Liver
Extracted molecule total RNA
Extraction protocol RNA was isolated from whole brain and liver by a commercial RNA extraction kit (RNAXEL, Eurobio), treated with Dnase and cleaned using RNeasy columns (Qiagen). Equal amount of total RNA was pooled from 3 mice within each of the 6 experimental groups (3 strains, 2 conditions = 6 ; in triplicate : 6 * 3 = 18 chips) for both brain and liver ( 36 chips in total).
Label biotin
Label protocol Target preparation were performed as described by the Affymetrix GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were conducted at the Genomics Platform of the NCCR Frontiers in Genetics at the University of Geneva (Switzerland) . Equal quantities of total RNA from three individual mice of each strain were pooled. We generated a hybridization mixture containing 15 ug of biotinylated cRNA according to the protocols provided by Affymetrix.
 
Hybridization protocol Fifteen micrograms of biotinylated cRNA from each sample were fragmented and hybridized to GeneChip Mouse 430 2.0 arrays, according to standard procedures. Washing and staining was performed using the EuGE-WS2v5 protocol.
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 7G.
Description none
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
 
Submission date Oct 26, 2007
Last update date Aug 28, 2018
Contact name Mehdi Tafti
E-mail(s) mehdi.tafti@unil.ch
Phone +41216923971
Organization name University of Lausanne
Department CIG
Street address Genopode
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platform ID GPL1261
Series (2)
GSE9441 The effect of sleep deprivation on gene expression in the brain and the liver of three inbred mouse strains
GSE9444 Sleep deprivation and the brain
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA; Log2 normalized expression signal

Data table
ID_REF VALUE
1415670_at 8.791852306
1415671_at 9.285794433
1415672_at 9.17033574
1415673_at 5.985636247
1415674_a_at 7.566682674
1415675_at 7.198770024
1415676_a_at 10.07294231
1415677_at 9.45363915
1415678_at 9.392488864
1415679_at 9.642872173
1415680_at 7.460267237
1415681_at 9.230246316
1415682_at 7.291917338
1415683_at 9.132335813
1415684_at 7.952471759
1415685_at 7.73471635
1415686_at 8.643166975
1415687_a_at 10.53177461
1415688_at 9.042207134
1415689_s_at 6.840825841

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM239853.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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