|
| Status |
Public on Jan 01, 2008 |
| Title |
wild-type tumor #3375 |
| Sample type |
RNA |
| |
|
| Source name |
PyV-mT wild-type tumor
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: female
Age: 110 days
Tissue: mammary tumor
Days after tumor onset: 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tumor tissue (100 mg) was homogenized in Ultraspec (Biotecx Labs, Houston, TX), and total RNA was extracted according to the manufacturer's protocol.
|
| Label |
Biotin (Affymetrix IVT)
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Murine Genome 430 2.0 Array GeneChip. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Six PyV-mT St6gal1 null and wild-type tumor pairs were used, in four independent pools of three tumors each.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 27, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Maria Hedlund |
| E-mail(s) |
mahedlund@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
9500 Gilman Dr CMM-E #1087
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0687 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9447 |
Alpha2-6-linked Sialic Acids on N-Glycans Modulate Carcinoma Differentiation In Vivo |
|
| Relations |
| Reanalyzed by |
GSE119085 |
