|
Status |
Public on Dec 01, 2007 |
Title |
PM_mouse_Lung OVA/PM rep3 |
Sample type |
RNA |
|
|
Source name |
Lung
|
Organism |
Mus musculus |
Characteristics |
A/J, male Age: 8-10 week
|
Biomaterial provider |
Charles Rivers Laboratories (Wilmington, MA)
|
Treatment protocol |
Oval albumin (0.4 mg/kg i.p, day 0) and an intratracheal OVA challenge (30 mg/kg, day 14); 50 µl aliquot of PM suspended in PBS (10 mg/ml) was placed in the back of the oral cavity and aspirated by the animal.
|
Growth protocol |
standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Gene expression data from lung tissue of control animal
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization;
|
|
|
Submission date |
Oct 30, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Yong Huang |
E-mail(s) |
yh9fj@virginia.edu
|
Phone |
(434) 243-0842
|
Organization name |
University of Viginia
|
Department |
Medicine
|
Street address |
1340 Jefferson Park Ave
|
City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9465 |
Murine Pulmonary Responses to Ambient Baltimore Particulate Matter |
|
Relations |
Reanalyzed by |
GSE119085 |