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Sample GSM240425 Query DataSets for GSM240425
Status Public on Oct 30, 2007
Title bc-ZT21-2
Sample type RNA
 
Source name prefrontal cortex
Organism Mus musculus
Characteristics prefrontal cortex
Extracted molecule total RNA
Extraction protocol The prefrontal cortex tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Analysis of prefrontal cortex from 3 biological replicates of C57BL/6J mice at four Zeitgeber Times (ZT 3, 9, 15, and 21).
Data processing Affymetrix Microarray Suite version 5.0 followed by GCRMA normalization by GeneSpring 7.2
 
Submission date Oct 30, 2007
Last update date Aug 14, 2011
Contact name Maja Bucan
E-mail(s) bucan@pobox.upenn.edu
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL1261
Series (1)
GSE9471 Diurnally regulated gene expression in the prefrontal cortex of C57Bl/6J mice at Zeitgeber Time (ZT) 3, 9, 15, and 21.

Data table header descriptions
ID_REF
VALUE GCRMA normalized MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 1054.9205 P 0.000732
1415671_at 2560.5715 P 0.000244
1415672_at 2704.0447 P 0.000244
1415673_at 484.5626 P 0.001221
1415674_a_at 669.33887 P 0.000732
1415675_at 623.99713 P 0.000244
1415676_a_at 2702.7195 P 0.000244
1415677_at 1072.3479 P 0.000244
1415678_at 1227.2178 P 0.000244
1415679_at 1142.9376 P 0.000244
1415680_at 274.94235 P 0.00293
1415681_at 412.0874 P 0.000244
1415682_at 385.482 P 0.001221
1415683_at 937.3195 P 0.000244
1415684_at 234.8066 P 0.000732
1415685_at 239.25331 P 0.000244
1415686_at 1731.0144 P 0.000732
1415687_a_at 5912.0034 P 0.000244
1415688_at 1268.192 P 0.000244
1415689_s_at 190.986 P 0.001953

Total number of rows: 45101

Table truncated, full table size 1400 Kbytes.




Supplementary file Size Download File type/resource
GSM240425.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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