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Sample GSM240735 Query DataSets for GSM240735
Status Public on Jan 17, 2008
Title Empty_Pt_A_3
Sample type RNA
 
Source name Patient sample 1 infected with a lentivirus expressing an empty vector
Organism Homo sapiens
Characteristics Patient sample 1 infected with a lentivirus expressing an empty vector
Treatment protocol shRNAs were introduced into normal CD34+ human hematopoietic progenitor cells,
Growth protocol CD34+ cells were induced to differentiate for 10 days along the erythroid and megakaryocytic lineages.
Extracted molecule total RNA
Extraction protocol RNA was purified from mononuclear cells using Trizol (Invitrogen).
Label biotin
Label protocol Linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labeling System (Nugen, Inc.).
 
Hybridization protocol cDNA was hybridized to HG_U133AAofAv2 microarrays following standard Affymetrix procedure.
Scan protocol Affymetrix
Description Patient sample 1 infected with a lentivirus expressing an empty vector
Data processing Raw expression values were normalized using Robust Multiarray Averaging (RMA)
 
Submission date Oct 31, 2007
Last update date Aug 14, 2011
Contact name Pablo Tamayo
E-mail(s) ptamayo@ucsd.edu
Phone 508-259-0630
Organization name UCSD
Department Moores Cancer Center
Street address 3855 Health Sciences Drive MC 0658
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL3921
Series (1)
GSE9487 Genome-wide expression profiling of cells infected with control or RPS14 shRNAs

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 66.3560738
1053_at 876.308985
117_at 65.26657795
121_at 95.86587201
1255_g_at 26.35163833
1294_at 205.3503677
1316_at 85.08146834
1320_at 45.41714129
1405_i_at 34.61036743
1431_at 36.51344225
1438_at 28.05169132
1487_at 340.3370402
1494_f_at 25.62904315
1598_g_at 113.8054242
160020_at 87.14646153
1729_at 140.7243523
1773_at 48.49640961
177_at 150.8990348
179_at 47.52393989
1861_at 90.47613518

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM240735.CEL.gz 2.8 Mb (ftp)(http) CEL
GSM240735.EXP.gz 330 b (ftp)(http) EXP
Processed data included within Sample table

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