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Sample GSM241196 Query DataSets for GSM241196
Status Public on Nov 04, 2008
Title 400mMsorbitolTSCJian24h rap 2
Sample type RNA
 
Source name mouse embryos
Organism Mus musculus
Characteristics embryos treated with 400mMsorbitol for 24h
Treatment protocol Mouse trophoblast stem cells were cultured in the same media in the ‘growth protocol’ except 400mM sorbitol was added to cause hyperosmolar stress. Cells were harvested for analysis by microarray at 5 time points from 0-24hr of stress.
Growth protocol Mouse placental trophoblast stem cells were cultured in standard media supplemented with conditioned media from mouse embryonic fibroblasts and 10ng/ml fibroblast growth factor (FGF)4 and heparin. The latter two are known to keep trophoblast stem cells in a undifferentiated pluripotent state.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cells 2-3 times for each time point. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Murine Genome 430.2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymatrix gene chip scanner 3000.
Description Gene expression data from Mouse embryos treated with 400mMsorbitol for 24h
Data processing All cel files were read and normalized by D-Chip software, normalized data were analysed by GeneSpring software
 
Submission date Nov 05, 2007
Last update date Aug 28, 2018
Contact name wenbo xu
Organization name wayne state university
Street address 3127 Scott Hall ,540 East Canfield
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (1)
GSE9519 The time-dependent response of placental trophoblast stem cells to hyperosmolar stress
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE D-Chip software
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 391.246 P
AFFX-BioB-M_at 476.478 P
AFFX-BioB-3_at 292.781 P
AFFX-BioC-5_at 692.118 P
AFFX-BioC-3_at 817.899 P
AFFX-BioDn-5_at 1784.42 P
AFFX-BioDn-3_at 2766.42 P
AFFX-CreX-5_at 6579.65 P
AFFX-CreX-3_at 6802.02 P
AFFX-DapX-5_at 100 A
AFFX-DapX-M_at 100 A
AFFX-DapX-3_at 100 A
AFFX-LysX-5_at 100 P
AFFX-LysX-M_at 100 A
AFFX-LysX-3_at 100 A
AFFX-PheX-5_at 100 A
AFFX-PheX-M_at 100 A
AFFX-PheX-3_at 100 A
AFFX-ThrX-5_at 100 A
AFFX-ThrX-M_at 100 A

Total number of rows: 45101

Table truncated, full table size 814 Kbytes.




Supplementary file Size Download File type/resource
GSM241196.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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