|
Status |
Public on Jun 15, 2008 |
Title |
Thymus, technical replicate 2 |
Sample type |
RNA |
|
|
Source name |
rhesus macaques thymus
|
Organism |
Macaca mulatta |
Characteristics |
Tissue: thymus
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
|
|
Hybridization protocol |
Following fragmentation, the biotinylated cRNA were hybridized for 16 hr at 45C on Rhesus Macaque Genome Array or Human Genome U133 Plus 2.0 Array
|
Scan protocol |
GeneChips were scanned on Affymetrix scanner 3000
|
Description |
Gene expression data from thymus
|
Data processing |
The data were preprossed with GC-Robust Multichip Analysis (RMA).
|
|
|
Submission date |
Nov 06, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Fenghai Duan |
E-mail(s) |
fduan@stat.brown.edu
|
Phone |
401-863-2175
|
Organization name |
Brown University
|
Department |
Biostatistics
|
Street address |
121 S. Main Street
|
City |
Providence |
State/province |
RI |
ZIP/Postal code |
02912 |
Country |
USA |
|
|
Platform ID |
GPL3535 |
Series (1) |
GSE9531 |
Rhesus Macaque gene expression data obtained using Rhesus Macaque Array or Human Genome U133 Plus 2.0 Array |
|