Small intestines were excised, homogenized in TRIzol reagent, whereafter purified total RNA was isolated using Qiagen RNEasy columns
Label
biotin
Label protocol
The RNA (5µg) was labled using the ENZO labeling kit, according to the manufacteror's instructions
Hybridization protocol
Arrays were hybridized according to the standard protocols of Affymetrix
Scan protocol
Arrays were scanned on an Affymetrix GeneChip scanner 7G, according to the standard protocols of Affymetrix
Description
Pure bred wild-type (129S1/SvImJ) and PPAR-null (129S4/SvJae) mice were treated for 6 hours with the synthetic triacylglycerols triolein [oleic acid (OA, C18:1)], trieicosapentaenoin [eicosapentaenoic acid (EPA, C20:5)] or tridocosahexaenoin [ocosahexaenoic acid (DHA, C22:6)], or the potent PPAR agonist WY14,643
Data processing
Expression estimates were calculated using GCRMA, applying the emperical Bayes estimate
