NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM241421 Query DataSets for GSM241421
Status Public on May 22, 2008
Title intestine_WT_WY_6hr_rep2
Sample type RNA
 
Source name small intestine complete, wild type, WY14643 treatment, 6hr
Organism Mus musculus
Characteristics 129S1/SvImJ, Male
Extracted molecule total RNA
Extraction protocol Small intestines were excised, homogenized in TRIzol reagent, whereafter purified total RNA was isolated using Qiagen RNEasy columns
Label biotin
Label protocol The RNA (5µg) was labled using the ENZO labeling kit, according to the manufacteror's instructions
 
Hybridization protocol Arrays were hybridized according to the standard protocols of Affymetrix
Scan protocol Arrays were scanned on an Affymetrix GeneChip scanner 7G, according to the standard protocols of Affymetrix
Description Pure bred wild-type (129S1/SvImJ) and PPAR-null (129S4/SvJae) mice were treated for 6 hours with the synthetic triacylglycerols triolein [oleic acid (OA, C18:1)], trieicosapentaenoin [eicosapentaenoic acid (EPA, C20:5)] or tridocosahexaenoin [ocosahexaenoic acid (DHA, C22:6)], or the potent PPAR agonist WY14,643
Data processing Expression estimates were calculated using GCRMA, applying the emperical Bayes estimate
 
Submission date Nov 06, 2007
Last update date Aug 28, 2018
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platform ID GPL1261
Series (1)
GSE9533 PPARalpha-mediated effects of dietary lipids on intestinal barrier gene expression
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA (EB) signal, version 2.20

Data table
ID_REF VALUE
1415670_at 9.949656304
1415671_at 9.302936771
1415672_at 9.722149464
1415673_at 6.999172891
1415674_a_at 8.768222077
1415675_at 7.530329232
1415676_a_at 9.562609058
1415677_at 11.85983014
1415678_at 10.60446742
1415679_at 10.86894252
1415680_at 8.711078285
1415681_at 9.154468474
1415682_at 7.746400525
1415683_at 9.972129791
1415684_at 8.324649284
1415685_at 7.920921068
1415686_at 9.016333653
1415687_a_at 8.546638559
1415688_at 9.692135715
1415689_s_at 7.977423561

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM241421.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap