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Status |
Public on Jan 08, 2018 |
Title |
Aldosterone-producing-adenoma_360 |
Sample type |
genomic |
|
|
Source name |
Aldosterone-producing adenoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: adenoma somatically mutated gene: KCNJ5
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated from APA and CPA tissues of the 42 patients using All Prep DNA/RNA Mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instruction.
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina labelling protocol
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|
|
Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol.
|
Scan protocol |
Beadchip arrays were imaged using the iScan system with standard recommended Illumina scanner setting.
|
Description |
APA360
|
Data processing |
Genome Studio V2011.2 (Illumina) Normalized average beta value. The methylation level of each of the > 850,000 CpG sites was calculated using the GenomeStudio Methylation Module 1.9.0 (Illumina). Normalization was performed using Normalization to Internal Controls and Background Subtraction options implemented in the Methylation Module 1.9.0. Unmethylated and methylated signal intensities in APA_EPIC_matrix-signal-intensities.txt supplementary file
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Submission date |
Dec 05, 2016 |
Last update date |
Jan 08, 2018 |
Contact name |
Kazuhiko Nakabayashi |
E-mail(s) |
nakabaya-k@ncchd.go.jp
|
Organization name |
National Research Institute for Child Health and Development
|
Department |
Department of Maternal-Fetal Biology
|
Street address |
2-10-1 Okura
|
City |
Setagaya |
State/province |
Tokyo |
ZIP/Postal code |
157-8535 |
Country |
Japan |
|
|
Platform ID |
GPL21145 |
Series (1) |
GSE90867 |
Molecular characteristics of the KCNJ5 mutated aldosterone-producing adenomas. |
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