|
| Status |
Public on Dec 12, 2007 |
| Title |
SOCS3 Cre plus C |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water.
|
| Biomaterial provider |
University of Washington
|
| Treatment protocol |
Hepatocyte-specific excision of the Socs3 gene was achieved by breeding Socs3 fl/fl mice with mice expressing the Cre recombinase transgene under control of the albumin promoter (Alb-Cre+), yielding Socs3 h-KO mice (SOCS3 Cre plus). Socs3 fl/fl, Alb-Cre- littermates were used as controls for all experiments, and are referred to as SOCS3 Cre minus. All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water. 2/3 partial hepatectomies (PH) were performed as previously described.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole liver tissue harvested 18h after PH, as described in Supplemental materials and methods.
|
| Label |
biotin
|
| Label protocol |
Total RNA (2 ug) was labeled and hybridized to the Mouse Genome 430 2.0 arrays and hybridized according to manufacurer’s instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water.
|
| Data processing |
Bioconductor GCRMA normalization log2
|
| |
|
| Submission date |
Nov 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9549 |
Regulation of Liver Regeneration and Hepatocarcinogenesis by Suppressor of Cytokine Signaling 3 |
|
| Relations |
| Reanalyzed by |
GSE119085 |
