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Sample GSM241743 Query DataSets for GSM241743
Status Public on Dec 12, 2007
Title SOCS3 Cre plus C
Sample type RNA
 
Source name liver
Organism Mus musculus
Characteristics All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water.
Biomaterial provider University of Washington
Treatment protocol Hepatocyte-specific excision of the Socs3 gene was achieved by breeding Socs3 fl/fl mice with mice expressing the Cre recombinase transgene under control of the albumin promoter (Alb-Cre+), yielding Socs3 h-KO mice (SOCS3 Cre plus). Socs3 fl/fl, Alb-Cre- littermates were used as controls for all experiments, and are referred to as SOCS3 Cre minus. All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water. 2/3 partial hepatectomies (PH) were performed as previously described.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from whole liver tissue harvested 18h after PH, as described in Supplemental materials and methods.
Label biotin
Label protocol Total RNA (2 ug) was labeled and hybridized to the Mouse Genome 430 2.0 arrays and hybridized according to manufacurer’s instructions.
 
Hybridization protocol The standard hybridization protocol was used as recommended by Affymterix.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
Description All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water.
Data processing Bioconductor GCRMA normalization log2
 
Submission date Nov 08, 2007
Last update date Aug 28, 2018
Contact name James William MacDonald
E-mail(s) jmacdon@uw.edu
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
 
Platform ID GPL1261
Series (1)
GSE9549 Regulation of Liver Regeneration and Hepatocarcinogenesis by Suppressor of Cytokine Signaling 3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA normalized signal log2

Data table
ID_REF VALUE
1415670_at 10.0440547193395
1415671_at 9.4561173481607
1415672_at 10.3171557968346
1415673_at 6.9406736259775
1415674_a_at 8.07048968008427
1415675_at 6.92217656694992
1415676_a_at 11.3036357144254
1415677_at 9.9348885528226
1415678_at 9.11536313682587
1415679_at 10.207971319738
1415680_at 7.44458998586137
1415681_at 9.01268267852819
1415682_at 6.83787278539816
1415683_at 9.93767842586823
1415684_at 8.29029929553192
1415685_at 8.33421759179293
1415686_at 8.5110868561991
1415687_a_at 10.7888915063904
1415688_at 9.25656581362373
1415689_s_at 5.26035629019431

Total number of rows: 45101

Table truncated, full table size 1245 Kbytes.




Supplementary file Size Download File type/resource
GSM241743.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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